Product Class: Other

Monarch® Spin Columns S2D and Tubes


Catalog #T1117

Product Introduction

  • Monarch spin columns and collection tubes designed for nucleic acid purification
  • Minimal to no buffer retention/risk of carry-over contamination
  • Unique column design enables a low elution volume (≥ 30 μl)
  • Made using less plastic compared to conventional columns from leading suppliers
  • Available for purchase separately, and included with Monarch Spin Nucleic Acid Purification Kits (NEB #T1110)

Product Information

Description

Monarch Spin Columns S2D and Tubes have been designed and assembled to deliver high performance for nucleic acid purification and concentration. These columns are designed without an O-ring, eliminating buffer retention and the risk of salt carry-over contamination, resulting in highly pure nucleic acid. The tapered column design allows elution volumes as low as 30 μl for highly concentrated nucleic acid suitable for various downstream applications. Additionally, Monarch spin columns are designed with sustainability in mind, using less plastic than conventional columns without compromising performance. These columns are compatible with centrifugation and vacuum manifold protocols and are supplied with collection tubes.

Properties
Also included as a component in: Monarch Spin Plasmid Miniprep Kit (NEB #T1110)
Product format: Spin columns and collection tubes for nucleic acid purification
Technology and purification format: Silica membrane-based purification technology
Provided as: 100 columns and 100 collection tubes
Suitable for: Plasmid purification
Nucleic acid binding capacity: 20 to 100 μg


FIGURE 1: Features of the Monarch Spin Column S2D for Plasmid and Nucleic Acid Purification

T1117 Column Desigggn

NEB® Monarch’s unique column design and membrane assembly allows high-quality nucleic acid purification with low elution volume, for highly-concentrated nucleic acid for downstream applications. The column is designed and made with significantly less plastic for a reduced environmental impact, while still maintaining structural integrity and enabling high performance.





This product is related to the following categories:
Plasmid Purification Products,
Nucleic Acid Purification Products,
This product can be used in the following applications:
Nucleic Acid Purification

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

FAQs & Troubleshooting

FAQs

  1. What is the smallest volume of elution buffer that can be used with Monarch® Spin Plasmid Miniprep Kit (NEB #T1110)?
  2. I have used 5 ml of cell culture for plasmid isolation with the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110), and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to improve yield and purity?
  3. Are the collection tubes part of the Monarch® Spin Column S2D and Tubes (NEB #T1117L) package, or do they have to be bought separately?
  4. I notice that I'm applying force to fit the column into a collection tube. Should I be doing this? What collection tubes are compatible with columns?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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