Product Information

Description

The Monarch Spin DNA Gel Extraction Kit offers an efficient method for DNA gel extraction and purification, enabling up to 5 μg of high-quality DNA. This new version features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 5 μl, for highly concentrated DNA in downstream applications such as sequencing, restriction digestion, library construction, labeling and other enzymatic manipulations. The kit includes spin columns, collection tubes, buffers, and other reagents in compact and streamlined packaging for silica membrane-based purification and DNA gel extraction. Our optimized gel dissolving buffer allows efficient gel dissolving and DNA binding on the column, without a need to monitor pH or add isopropanol. These kits contain uniquely designed columns and bottles to significantly reduce plastic usage compared to leading suppliers, while maintaining structural integrity for centrifugation, vacuum manifolds and other steps. The kit features Monarch spin columns that are manufactured using state of the art automation machinery for high quality and consistency.

Properties
Purification format	Spin column for nucleic acid purification
Compatible methods	Centrifugation or vacuum manifold
DNA sample	dsDNA from agarose gels
Typical recovery	70-90% for < 10 kb and 50-70% for ≥ 10 kb
DNA Purity	A260/280 > 1.8 and A260/230 > 1.8
Binding Capacity	Up to 5 μg
Elution Volume	≥ 5 μl
DNA Size Range	Standard protocol: 100 bp – 25 kb
Protocol Time	10 - 15 minutes of spin and incubation time
Compatible Downstream Applications	Ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing

 

Figure 1: The Monarch Spin DNA Gel Extraction Kit consistently recovers DNA as well as or better than the leading supplier, and is more concentrated for greater downstream utility

Figure 2: DNA purified from agarose gels using Monarch Spin DNA Gel Extraction Kit yields high-quality DNA, suitable for downstream applications

Figure 3: Monarch Spin DNA Gel Extraction Kit is effective for a wide range of DNA sizes

Figure 4: Features of the Monarch Spin Column S1A

Figure 5: Overview of the Monarch Spin DNA Gel Extraction Kit workflow

This product is related to the following categories:

DNA Gel Extraction Products,

Nucleic Acid Purification Products,

This product can be used in the following applications:

Nucleic Acid Purification

Features

• High Performance: Achieve high yields (up to 5 μg) and high purity in the extraction and purification of DNA from agarose gels, with the capability to remove contaminants and residual salts.
• High Concentration: Elute in very small volumes, in as little as 5 μl for elution, allowing for highly concentrated DNA.
• Time Savings: A short protocol of only 10 minutes of spin and incubation time is needed to complete the workflow.
• Unique Design: Spin column features a unique design that enables elution in low volumes and minimizes buffer retention and contaminant carryover.
• Optimized: Buffer system is optimized, without the need to adjust pH or add isopropanol.
• Application Compatibility: Purified DNA is ready for downstream molecular applications, such as restriction digests, DNA sequencing, ligation, amplification, and other enzymatic reactions.

Protocols, Manuals & Usage

FAQs & Troubleshooting

Quality, Safety & Legal