Product Information

Description

The Monarch Spin PCR & DNA Cleanup Kit (5 μg) offers an efficient method for purification and concentration up to 5 μg of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription. This new version features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 5 μl, for highly concentrated DNA in downstream applications such as sequencing, restriction digestion, library construction, labeling and other enzymatic manipulations. The kit includes spin columns, collection tubes, buffers, and other reagents in compact and streamlined packaging for silica membrane-based PCR purification and DNA cleanup. Our optimized buffer system allows efficient binding and purification of the DNA, without a need to monitor pH. These kits contain uniquely designed columns and bottles to significantly reduce plastic usage compared to leading suppliers, while maintaining structural integrity for centrifugation, vacuum manifolds and other steps. The kit features Monarch spin columns that are manufactured using state of the art automation machinery for high quality and consistency.

Properties
Purification format	Spin-column
DNA sample	DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations)
	ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol
Typical recovery	70-90%
DNA Purity	A260/280 > 1.8 and A260/230 > 1.8
Binding Capacity	Up to 5 μg
Elution Volume	5-20 μl
DNA Size Range	Standard protocol: 50 bp – 25 kb
Protocol Time	5 minutes of spin and incubation time
Compatible Downstream Applications	Ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing

 

Figure 1: Monarch Spin PCR & DNA Cleanup Kit (5 μg) consistently produces more concentrated DNA, with equivalent or better DNA recovery and purity compared to the leading supplier

Figure 2: DNA purified using Monarch® Spin PCR & DNA Cleanup Kit (5 μg) are high-quality and suitable for downstream applications

 

Figure 3: Monarch Spin PCR & DNA Cleanup Kit (5 μg) effectively cleans up oligonucleotide DNA using the oligonucleotide cleanup protocol.

Figure 4: Features of the Monarch Spin Column S1A

Figure 5: Overview of the Monarch Spin PCR & DNA Cleanup Kit (5 μg) workflow

This product is related to the following categories:

DNA Cleanup Products,

Nucleic Acid Purification Products,

This product can be used in the following applications:

Nucleic Acid Purification

Features

• High Performance: Achieve high yields (up to 5 μg) and high purity in the purification, cleanup, and concentration of DNA, with the capability to remove short primers, detergents, and other low-molecular-weight reaction components (e.g., nucleotides, DMSO, betaine).
• High Concentration: Elute in very small volumes, in as little as 5 μl for elution, allowing for highly concentrated DNA.
• Powerful Flexibility – Purify small DNA fragments, including oligonucleotides, with this single kit, using the modified protocol provided.
• Time Savings: Short protocol of only 5 minutes to complete the workflow of bind, wash, and elution steps, including minimal incubation and spin time.
• Unique Design: Spin column features a unique design that enables elution in low volumes and minimizes buffer retention and contaminant carryover.
• Optimized: Buffer system is optimized, without the need to adjust pH.
• Application Compatibility: Purified DNA is ready for downstream molecular applications, such as restriction digests, DNA sequencing, ligation, amplification, and other enzymatic reactions.

Protocols, Manuals & Usage

FAQs & Troubleshooting

Quality, Safety & Legal